This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jackson, J B
Right arrow Articles by Balfour, H H
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jackson, J B
Right arrow Articles by Balfour, H H, Jr

 Previous Article

Clin Microbiol Rev. 1988 January; 1(1): 124-138

Practical diagnostic testing for human immunodeficiency virus.

J B Jackson and H H Balfour Jr

Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis 55455.

SUMMARY

Since the discovery of human immunodeficiency virus (HIV) as the causative agent of acquired immunodeficiency syndrome in 1983, there has been a proliferation of diagnostic tests. These assays can be used to detect the presence of HIV antibody, HIV antigen, HIV ribonucleic and deoxyribonucleic acids, and HIV reverse transcriptase. Enzyme-linked immunosorbent assays, Western blot, radioimmunoprecipitation assays, indirect immunofluorescence assays, reverse transcriptase assays, and several molecular hybridization techniques are currently available. Enzyme-linked immunosorbent, Western blot, and indirect immunofluorescence assays for HIV antibody are very sensitive, specific, and adaptable to most laboratories. An enzyme-linked immunosorbent assay for HIV antigen is also readily adaptable to most laboratories and will be commercially available soon. While the other assays are more tedious, they are valuable confirmatory tests and are suitable for reference laboratories. The biohazards of performing HIV testing can be minimized with proper biosafety measures.

Clin Microbiol Rev. 1988 January; 1(1): 124-138




This article has been cited by other articles:

  • Reichelderfer, P S, Jackson, J B (1994). Quality assurance and use of PCR in clinical trials.. Genome Res 4: S141-S149 [Abstract]  
  • Brandt, C. D., Rakusan, T. A., Sison, A. V., Saxena, E. S., Ellaurie, M., Sever, J. L. (1994). Human Immunodeficiency Virus Infection in Infants During the First 2 Months of Life: Reliable Detection and Evidence of In Utero Transmission. Arch Pediatr Adolesc Med 148: 250-254 [Abstract]  
  • O'Brien, T. R., George, J. R., Holmberg, S. D. (1992). Human Immunodeficiency Virus Type 2 Infection in the United States: Epidemiology, Diagnosis, and Public Health Implications. JAMA 267: 2775-2779 [Abstract]  
  • Allen, U. D., Guerriere, M., Read, S. E., Detsky, A. S. (1991). Percutaneous Injuries Among Health Care Workers: The Real Value of Human Immunodeficiency Virus Testing of 'Donor' Blood. Arch Intern Med 151: 2033-2040 [Abstract]  
  • Benenson, A. S., Peddecord, K. M., Hofherr, L. K., Ascher, M. S., Taylor, R. N., Hearn, T. L. (1989). Reporting the Results of Human Immunodeficiency Virus Testing. JAMA 262: 3435-3438 [Abstract]  
  • (1989). Update: HIV-2 Infection--United States. JAMA 262: 1579-1583  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»